Plasmid Modification by Annealed Oligo Cloning - Addgene
Ligation Dilute 5μL of annealed oligos with 45μL nuclease-free water and quantify the concentration (should be about 8ng/μl). Mix the annealed oligos with cut vector in molar ratios …
第二代DNA测序原理 - SOLiD 测序技术 - 知乎 - 知乎专栏
SOLiD 的全称为：Sequencing by Oligo Ligation Detection，是ABI公司与2007年推出的 测序平台 。 SOLiD测序平台以四色荧光标记 寡核苷酸 的连续合成为基础，取代了传统的聚合酶连接反 …
Template-dependent DNA ligation for the synthesis of modified
Sep 13, 2024 · Herein, we report a robust alternative method for the de novo synthesis of modified oligonucleotides using template-dependent DNA ligation of shortmer fragments. Our …
Efficient assembly of very short oligonucleotides using T4 DNA …
We demonstrate the feasibility of simultaneous oligonucleotide phosphorylation and ligation and, as a proof of principle for DNA synthesis through the assembly of short oligonucleotides, we …
Annealing Oligonucleotides Protocol | IDT - Integrated DNA Technologies
Jun 15, 2012 · Ligation—if the double-stranded oligonucleotide product will be used in a ligation reaction, you may need to add 5’-phosphates to the strand ends. These can be added at the …
Highly Parallelized Construction of DNA from Low-Cost Oligonucleotide …
Feb 20, 2024 · We propose an improvement on gene construction synthesis schemes using oligonucleotide (oligo) pools as sources of user-defined DNA sequences for decentralized …
Oligo annealing for ligation cloning — Galloway Lab Protocols …
Jun 8, 2023 · This protocol describes how to anneal and phosphorylate oligos to add short sequences to plasmids via ligation cloning. This could be useful to make new microRNA target …
A ligation-based single-stranded library preparation method to …
Dec 27, 2019 · Using synthetic DNA oligos and cfDNA, we demonstrate the efficiency and utility of this approach and compare with existing double-stranded and single-stranded approaches …
Ligation of a DNA or RNA oligo to the 3’ end of long ssRNA
Oct 27, 2014 · This protocol is for ligation of a DNA or RNA oligo donor with a 5'P to the 3'OH end of long ssRNA (> 0
Biocatalytic assembly of chemically modified oligonucleotides
Mar 16, 2022 · Inspired by a well-established biological construction of DNA and RNA strands [9], [10], [11], a therapeutic oligonucleotide can be split into two or more, shorter fragments, which …