Whole mount immunofluorescent staining of the neonatal mouse retina …
2013年7月9日 · This article demonstrates a straightforward procedure for analyzing the vasculature of a mouse retina using fluorescent staining with isolectin and vascular specific antibodies. Angiogenesis is the complex process of new blood vessel formation defined by the sprouting of new blood vessels from a pre-existing vessel network.
[Abstract] Here we provide a detailed protocol for whole mount staining of mouse retina. This protocol was used to analyze retinal angiogenesis in newborn mice (Sawaguchi et al., 2017) by modifying the original protocols (Powner et al., 2012; Tual-Chalot et al., 2013. This protoco) l can also be usedfor whole mount staining of adult retina.
Non-separate Mouse Sclerochoroid/RPE/Retina Staining and …
2021年1月1日 · Here, we describe steps of both non-pigmented and pigmented mouse Sclerochoroid/RPE/Retina whole mount including eyeball preparation, staining, mounting and confocal scanning. In addition, we present the confocal images of RPE, subretinal microglia and the neighboring photoreceptors in Sclerochoroid/RPE/Retina whole mounts.
Spatial organization of the mouse retina at single cell ... - Nature
2023年8月15日 · Here, we generated the single-cell spatial atlas of the mouse retina using multiplexed error-robust fluorescence in situ hybridization (MERFISH). We profiled over 390,000 cells and identified...
1. Using a cocktail stick, gently pick up the retina and place on glass slide. Ensure that the retina side is facing up (For mouse the underside will be black). 2. Flatten out the retina on each slide, taking care to remove any debris, using pipette gently remove all liquid from the flat retina (this should ensure the retina straightens out). 3.
Step-by-step preparation of mouse eye sections for routine …
On those tissue sections, the round shape of the mouse eyeball, anterior and posterior chambers (Figure 2A), delicate structures such as the ciliary body (CB) and the trabecular meshwork (TM) region (Figure 2B), peripheral retina (Figure 2C), iris and anterior lens (Figure 2D), near-central retina (Figure 2E), central cornea (Figure 2F) and ...
Development of the outer retina in the mouse - ScienceDirect
2003年10月10日 · In this study, we describe the expression of markers for photoreceptors (recoverin), horizontal cells (calbindin), bipolar cells (protein kinase C; PKC) and cytoskeletal elements pivotal to axonogenesis (beta-tubulin and actin) …
Comprehensive single-cell atlas of the mouse retina - Cell Press
2024年6月21日 · To construct a comprehensive unified single-cell reference of the mouse retina, we generated 16 unpublished scRNA-seq samples of the mouse retina and incorporated four curated public datasets to enhance specific amacrine, bipolar and retinal ganglion cells.
Quick and reliable method for retina dissociation and …
2015年1月1日 · Thus, staining with anti-CD73 antibody represents a possible way to separate rod cells from retina of adult mice. The advent of powerful high throuput methods (e.g., RNA-seq, proteomics and ChiP-seq) now allows for a more detailed genomic and epigenomic analysis of physiological and pathophysiological processes of the retina.
Retinal Angiogenesis Regulates Astrocytic Differentiation in …
2017年12月14日 · In mice, retinal vascular and astrocyte networks begin to develop at birth, expanding radially from the optic nerve head (ONH) towards the retinal periphery. The retinal vasculature grows...