Immunohistochemistry is a powerful tool for visualizing the distribution and localization of antigens in tissue sections. Immunohistochemistry (IHC) uses antibodies to detect the location of proteins and
Immunohistochemistry for Pathologists: Protocols, Pitfalls ...
2016年8月8日 · Immunohistochemistry (IHC) is an important auxiliary method for pathologists in routine diagnostic work as well as in basic and clinical research including exploration of biomarkers, as IHC allows confirmation of target molecule expressions in the context of microenvironment.
The following guide outlines the general IHC protocol, as well as some useful tips and hints on how to optimize each step. Factors to be considered when designing an IHC experiment:
Immunohistochemistry Protocols | Thermo Fisher Scientific - US
Our staining kits are provided with complete protocols for use. This page lists the steps involved in using selected Histostain™ kits and the time required for each step. It also contains protocols for removing endogenous peroxidase, digesting tissue and antigen recovery.
When submerging, do not rapidly plunge the samples multiple times. The dunking motion can cause the sample to be washed off the cover slide. The time is optimized to allow for diffusion of each reagent. The best way to add slides to the coverplates is by submerging them both under water and pressing the slide to the coverplate.
IHC Immunohistochemistry (IHC) is a technique commonly used for morphological characterization of tumors or other tissue malignancies. This guide highlights critical steps in the IHC protocol and demonstrates how protocol changes can affect the final outcome of your experiment. A GUIDE TO SUCCESSFUL IMMUNOHISTOCHEMISTRY
Immunohistochemistry Procedure - MilliporeSigma
Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.