Fluorescein amidite - Wikipedia
Fluorescein amidites, abbreviated as FAM, are important synthetic equivalents of fluorescein dye used in oligonucleotide synthesis and molecular biology. FAM is used in the preparation of fluorescein-labeled oligonucleotide probes for the detection of the presence of the complementary nucleic acids or primers for polymerase chain reaction .
CRISPR-based diagnostics - Nature Biomedical Engineering
Jul 16, 2021 · In this technique, reporter molecules carrying biotin and fluorescein (fluorescein amidite (FAM)) bind to anti-fluorescein isothiocyanate (FITC) antibodies coupled to gold nanoparticles in the ...
Fluorescein - Wikipedia
Fluorescein is an organic compound and dye based on the xanthene tricyclic structural motif, formally belonging to triarylmethine dyes family. It is available as a dark orange/red powder slightly soluble in water and alcohol. ... Fluorescein amidite (FAM), synthetic equivalents of fluorescein used in oligonucleotide synthesis; Merbromin, or ...
What is FAM fluorescence? - SyronOptics
FAM fluorescence refers to the fluorescent properties of a dye known as Fluorescein Amidite (FAM). This dye is widely used in molecular biology and biochemistry for various applications, including DNA sequencing, qPCR, and fluorescence microscopy. FAM is known for its high quantum yield and its ability to emit a strong green fluorescence when ...
5'-Fluorescein CE-Phosphoramidite (6-FAM) - Biosearch …
Fluorescein-CE Phosphoramidite has a thiourea linkage which mimics the original method of incorporating fluorescein to an amino-modified oligo. It must be noted however that the linkage is attached via the 5 position of the ring system in this case. Internal sequence additions of Fluorescein are achieved using Fluorescein-dT.
Fluorescein Amidites and Supports | Application Notes
Fluorescein-dT Amidite: Fluorescein-dT has maximum absorbance at λ 494 nm and emission at λ 521 nm. Fluorescein-dT is a deoxythymidine nucleoside derivatized with 6-FAM (6-carboxyfluorescein) through a spacer arm (Figure 1, CLP-9905). Fluorescein-dT is used to internally label an oligonucleotide at a dT position.
A review on derivatives of fluorescein aldehydes and their applications
Jan 1, 2022 · The utilization of fluorescein amidite, appeared underneath right, permits one to blend marked oligonucleotides for a similar reason. One more strategy named molecular beacons utilizes fluorescein-named oligonucleotides. Fluorescein-marked tests can be imaged by utilizing FISH (Fluorescence in-situ hybridization), or focused by antibodies ...
Fluorescein—painting the Chicago river green! | IDT
Jun 17, 2011 · One common modification attached to oligos is the bright green fluorophore known as Fluorescein, FITC, or FAM (Fluorescein amidite). Attaching the fluorophore to oligonucleotides is not its only use though. Read on. Print Page. The Chicago River on St. Patrick’s Day 2009. ...
Energy Transfer Between i‐Motif DNA ... - Wiley Online Library
Subsequently, to enhance the sensitivity of ROS detection, we integrated the strong and stable fluorophores fluorescein amidite (FAM) and Cyanine 5 Cy5 with the ROS-sensory DNA/AgNCs. Through this approach, we achieved yet undefined energy transfer (ET) between FAM and AgNCs, resulting in the emission of highly stable green fluorescence in ...
Fluorescein amidite - chemeurope.com
Fluorescein amidite, abbreviated FAM, is an important dye used in molecular biology. It is often used in creation probes to detect the presence of specific nucleic acid sequences. A primer can be modified at one end with a FAM and at the other end with a quencher. The primer is designed such that its native state is a hairpin, allowing the ...