Current methods to analyze lysosome morphology, positioning, …
4.2.4 Correlative light electron microscopy. Lysosomes are highly dynamic organelles and while EM can capture lysosomal positioning, live cell light microscopy of fluorescent probes or ectopically expressed/gene-edited fluorescent lysosomal proteins is ideally suited for studying lysosome dynamics.
Molecular Expressions Cell Biology: Animal Cell Structure - Lysosomes
2015年11月13日 · After estimating the probable size of the lysosome, he was able to identify the organelle in images produced with an electron microscope. Lysosomes are found in all animal cells, but are most numerous in disease-fighting cells, such as white blood cells.
Lysosomes Dr.Jastrow's electron microscopic atlas
Lysosomes are present in all cells of animals with exception of red blood cells (erythrocyten). Their number is high in microphages (e.g. neutrophilic granulocytes ) and macrophages as well as in other phagocyting cells of the reticulohistiocytic and reticuloendothelial systems.
Methods for monitoring lysosomal morphology - PubMed
Here we describe methods for monitoring lysosome morphology in fixed and live cells using fluorescent probes and electron microscopy. Keywords: Electron microscopy; Endocytosis; Fluorescence microscopy; Immunofluorescence; LAMP1; Lysosome; Membrane traffic; NAADP; Two-pore channels.
Measuring lysosomal size and frequency by electron microscopy
2021年1月1日 · Electron microscopy (EM) is unsurpassed in high-resolution imaging of both ultrastructural and immunocytochemical features of subcellular compartments. EM-based morphometry permits precise quantitative analyses of organelles, especially after state-of-the-art cryopreparation.
Analyzing Lysosome-Related Organelles by Electron Microscopy
Deciphering the biogenesis and functions of lysosomes and lysosome-related organelles (LROs) and their dysfunctions requires their visualization and detailed characterization at high resolution by electron microscopy. Here, we provide detailed protocols for studying LROs by transmission electron microscopy.
Lysosomes: Current Biology - Cell Press
2015年4月20日 · Electron microscopy and the use of acid phosphatase cytochemistry later established the morphological identity of lysosomes. How do they function in membrane trafficking pathways? Lysosomes interact with late endosomes (also known as multivesicular bodies or MVBs) by direct fusion or ‘kiss-and-run’ events.
Lysosomes: fusion and function | Nature Reviews Molecular Cell …
Electron microscopy, which was not used in their initial discovery, subsequently showed that lysosomes constitute up to 5% of the intracellular volume and are of heterogeneous size and...
Systematic Transmission Electron Microscopy‐Based …
2023年3月3日 · Knockout of dynamin-related protein (DRP-1) increases lysosomes and autophagosomes, as identified through TEM, lysotracker, lysosome-associated membrane protein (LAMP1) immunofluorescence, and serial block face-scanning electron microscopy (SBF-SEM) 3D reconstruction.
Functional characterization of endo-lysosomal compartments by ...
2023年1月1日 · Here we introduce “functional correlative microscopy” (functional CLEM) to directly infer functional information from live cells to EM with nanometer resolution. We label and visualize live cells with fluorescent biosensors after which they are processed for EM and imaged using a volume electron microscopy technique.