Amine-Reactive Probe Labeling Protocol - Thermo Fisher Scientific
Molecular Probes manufactures a wide variety of amine-reactive fluorescent dyes, biotins, and other haptens for conjugation to proteins, amine-modified oligonucleotides, or other amine-containing compounds.
Labeling of Amino-Modified Oligonucleotides with Dye NHS esters
Purification of the Dye-Oligonucleotide Conjugate: The labeled oligonucleotide is first purified by repetitive ethanol precipitation as described above (see Note 3). The final purification is done by HPLC-separation on a reversed phase (C-18) column using an acetonitrile/H 2 …
Protocol for Conjugating NHS-Ester Modifications to Amino …
This protocol is for conjugating NHS-ester modifications, such as TxRd (Sulforhodamine 101-X) (protocol is effective for most NHS-ester modifications), to an oligonucleotide with an amino label, such as 5'-Amino-Modifier C6 (Figure 1).
Protocol to generate, purify, and analyze antibody-oligonucleotide ...
2024年12月20日 · Antibody-oligonucleotide conjugates (AOCs) are a fast-expanding modality for targeted delivery of therapeutic oligonucleotides to tissues. Here, we present a protocol to generate, purify, and analyze AOCs from off-the-shelf antibodies.
easy method which can be used to produce antibody-oligonucleotide conjugates for different kinds of applications, including mono-, double-, or triple-conjugates. synthesis of oligonucleotide -conjugated antibodies is based on the copper using a …
Enzymatic and Chemical Labeling Strategies for Oligonucleotides
2021年10月1日 · Oligos conjugated with fluorophores can readily be detected in assays involving either gel- or microplate readers, while oligos conjugated with biotin or resin are especially useful for affinity purification procedures.
Pacific Orange Fluorescent Dye Oligonucleotide Labeling - Bio-Syn
Bio-Synthesis offer Pacific Orange fluorescent dye oligonucleotide labeling services. Pacific Orange oligo conjugates has peak excitation wavelength at 405 and emission wavelength in 551 nm. This allows it to be used with other violet-excitable fluorophores such as Pacific Blue™ dye for multiparameter analysis using a single excitation source.
There are few factors that need be considered when SE compounds are used for conjugation reaction: . Solvents: For the most part, reactive dyes are hydrophobic molecules and should be dissolved in anhydrous dimethylformamide (DMF) or dimethylsulfoxide (DMSO).
Our Tide FluorTM dyes have stronger fluorescence and higher photostability than the classic fluorophores (such as fluoresceins, rhodamines and cyanines). They are the best affordable fluorescent dyes for labeling peptides and oligonucleotides without comprised performance.
Labeling Oligonucleotides and Nucleic Acids—Section 8.2
Oligonucleotide conjugates of the BODIPY FL, BODIPY R6G, BODIPY 564/570 and BODIPY FL, BODIPY R6G, BODIPY 564/570 and BODIPY 581/591 dyes have been found to be particularly useful for automated DNA sequencing.