Confocal microscopy - University of California, Berkeley
The Primary Dichroic Mirror reflects lower wavelength laser light onto the sample. The upper Mirror (past the pinhole) serves to transmit emitted light from the sample laterally to the detectors for multiple channel imaging.
二向色镜的介绍 - 知乎 - 知乎专栏
2020年9月1日 · 指45度入射或大角度入射时，把光源分离出特定的光谱改变部分光谱光路方向，常用于酶标仪器、荧光显微镜系统、投影光引擎系统、激光灯、光学仪器分束、视频眼镜等传感器系统。 是光学设计者狂热追求的光学元件。 能有效缩小器件的结构实现多功能的光路。 特点： 其特点是对一定波长的光几乎完全透过，而对另一些波长的光几乎完全反射。 激埃特二向分色镜具有高穿透率，波长定位准确，光能量损耗小等优点。 参数： 二向色镜曲线. 窄带滤光片常规应用： …
With a confocal LSM and it's variable pinhole it is therefore possible to exclusively image a thin optical slice out of a thick specimen (typically, up to 100 μm), a method known as optical sectioning. Under suitable conditions, the thick-ness (Z …
Any Way You Slice It—A Comparison of Confocal Microscopy …
In-focus light is selected by the pinhole aperture. The secondary dichroic mirror splits the green emission light and directs it to be detected by PMT1, and the red emission light passes and is detected by PMT2.
In recent years, the confocal Laser Scanning Micro-scope (LSM) has become widely established as a research instrument. The present brochure aims at giving a scientifically sound survey of the special nature of image forma-tion in a confocal LSM.
Dichroic coherent diffractive imaging - PNAS
2011年8月8日 · Here we present a novel approach to lensless imaging of an extended magnetic nanostructure, in which a scanned series of dichroic coherent diffraction patterns is recorded and numerically inverted to map its magnetic domain configuration. Unlike holographic methods, it does not require a reference wave or precision optics.
Introduction - W. M. Keck Observatory
Dichroic beam splitters invariably produce low frequency ``wiggles'' in the efficiency of transmitted and reflected light that can be noticed in high S/N spectral observations; these can be removed through flat fielding. The dichroic substrates are 1-inch thick BK7 glass, and are …
Dichroism - Wikipedia
In optics, a dichroic material is either one which causes visible light to be split up into distinct beams of different wavelengths (not to be confused with dispersion), or one in which light rays having different polarizations are absorbed by different amounts.
Dichroism – wavelength-dependent transmission, absorption, …
The term dichroism (with the corresponding adjective dichroic) is used with two different meanings, explained in the following sections. Wavelength-dependent Transmission. The term can be used for optical elements which somehow act on light with a substantial dependence on the optical wavelength.
What is the dichroic effect? – SyronOptics
The dichroic effect refers to the phenomenon where a material displays two different colors when viewed from different angles or under different lighting conditions. This effect is a result of the material's unique optical properties, which cause it to selectively absorb and transmit light of different wavelengths depending on the angle of ...