MicroRNA sequencing - Wikipedia
MicroRNA sequencing (miRNA-seq), a type of RNA-Seq, is the use of next-generation sequencing or massively parallel high-throughput DNA sequencing to sequence microRNAs, also called miRNAs. miRNA-seq differs from other forms of RNA-seq in that input material is often enriched for small RNAs. miRNA-seq allows researchers to examine tissue ...
Small RNA Sequencing | Small RNA and miRNA profiling and
Small RNA sequencing (RNA-Seq) is a technique to isolate and sequence small RNA species, such as microRNAs (miRNAs). Small RNA-Seq can query thousands of small RNA and miRNA sequences with unprecedented sensitivity and dynamic range.
miRBase
miRBase: the microRNA database the archive for microRNA sequences and annotations More about miRBase →
Protocols for the analysis of microRNA expression, biogenesis and ...
In the following protocols, we have included methods for microRNA isolation and small RNA or microRNA sequencing to allow expression profiling of microRNA during immune regulation. We have also included protocols for mapping Drosha cleavage sites …
Small RNA-Sequencing: Approaches and Considerations for miRNA …
2021年5月27日 · Small RNA-seq enables genome-wide profiling and analysis of known, as well as novel, miRNA variants. Moreover, its high sensitivity allows for profiling of low input samples such as liquid biopsies, which have now found applications in diagnostics and prognostics.
Single-cell microRNA sequencing method comparison and
2021年7月14日 · Molecular single cell analyses provide insights into physiological and pathological processes. Here, in a stepwise approach, we first evaluate 19 protocols for single cell small RNA sequencing on...
MicroRNA profiling: approaches and considerations - Nature
2012年4月18日 · Three main approaches are currently well established for miRNA profiling: quantitative reverse transcription PCR (qRT-PCR), hybridization-based methods (for example, DNA microarrays) and...
Direct microRNA Sequencing Using Nanopore-Induced Phase …
2020年3月27日 · Nanopore-induced phase-shift sequencing (NIPSS), which is a variant form of nanopore sequencing, could directly sequence any short analytes including miRNA. In practice, NIPSS clearly discriminates between different identities, isoforms, and epigenetic variants of model miRNA sequences.
Single-cell microRNA-mRNA co-sequencing reveals non-genetic ... - Nature
2019年1月9日 · Here, we present co-sequencing of microRNAs and mRNAs in the same single cell using a half-cell genomics approach. This method demonstrates good robustness (~95% success rate) and reproducibility...
The Study of Small RNA Sequencing from Biological Samples (miRNA …
2024年11月16日 · Studying miRNA is important as their deregulation has been shown to play important roles in cancer, HIV, and other diseases [10, 11]. Next-generation sequencing (NGS) of miRNAs is a useful technique to determine miRNA sequence and quantity of known and novel miRNAs, which were discovered to be involved in the pathophysiology of several diseases.