What are oligos? More information at LubioScience
2023年2月28日 · Oligos (or oligonucleotides) are short pieces of synthetic DNA or RNA — generally 20 to 100 nucleotides (nts) in length — although longer oligos serve specific functions. Employed in a myriad of applications, oligos are essential for next-generation sequencing (NGS), PCR cloning and diagnostic screening of diseases.
3D mapping and accelerated super-resolution imaging of the …
Here, we push toward this goal by introducing OligoFISSEQ, a suite of three methods that leverage fluorescent in situ sequencing of barcoded Oligopaint probes to enable the rapid visualization of many targeted genomic regions.
Single-molecule super-resolution imaging of chromosomes and
2015年5月12日 · Here the authors combine oligo-based DNA FISH with single-molecule super-resolution microscopy to image single-copy genomic regions and, taking advantage of SNPs, distinguish allelic regions of...
Oligonucleotide - Wikipedia
Oligonucleotides are short DNA or RNA molecules, oligomers, that have a wide range of applications in genetic testing, research, and forensics.
What is an oligo? | IDT - Integrated DNA Technologies
2023年4月14日 · Oligonucleotides, or oligos for short, are among the most important tools used in modern molecular biology, and they help fuel today’s biotechnology, pharmaceutical, medical research, and industries. But simply saying oligos are synthetic DNA or RNA oversimplifies just what they do and why they are important. What are oligos?
SgRNA and lentiCRISPRv2 - 简书
阴性对照：使用ddH 2 O代替Oligos. 转化. 使用Stbl3感受态. 慢病毒转染质粒包含Long-TerminalRepeats (LTRs) ，感受态细胞选择重组缺陷型的细菌以提高转化效率 。
secondary oligonucleotides (oligos) to produce and enhance fluorescent signals. These advances should substantially expand the capability to query parent-of-origin-specific chromosome positioning and gene expression on a cell-by-cell basis.
In situ imaging and isolation of proteins using dsDNA …
2004年11月23日 · In this paper we describe a method for the in situ detection and isolation of proteins that can be used to successfully image nuclear antigens by LM and EM. This method is based on the ability of a peptide tag to bind a double-stranded DNA oligonucleotide (dsDNA oligo) with high affinity.
Efficient and highly amplified imaging of nucleic acid targets in ...
2024年11月15日 · Here, we introduce a unified technical platform, termed ‘pSABER’, for the amplification of ISH signals in cell and tissue systems. pSABER decorates the in situ target with concatemeric binding...
Genome Oligopaint with oPools Oligo Pools - IDT
2021年7月21日 · Essentially, Oligopaints is a flexible, oligo-based technique that uses FISH to paint details, from single copy regions to megabases [2]. This tool has become increasingly powerful since it takes DNA from flow-sorted or micro …