Plasmid Modification by Annealed Oligo Cloning - Addgene
Ligation Dilute 5μL of annealed oligos with 45μL nuclease-free water and quantify the concentration (should be about 8ng/μl). Mix the annealed oligos with cut vector in molar ratios (vector:insert) between 4:3 and 1:6 in a standard ligation reaction (ex. to ligate an annealed oligo insert of 50bp in length into a 5kb vector, mix 100ng of the ...
第二代DNA测序原理 - SOLiD 测序技术 - 知乎 - 知乎专栏
SOLiD 的全称为：Sequencing by Oligo Ligation Detection，是ABI公司与2007年推出的 测序平台 。 SOLiD测序平台以四色荧光标记 寡核苷酸 的连续合成为基础，取代了传统的聚合酶连接反应。
Template-dependent DNA ligation for the synthesis of modified
2024年9月13日 · Herein, we report a robust alternative method for the de novo synthesis of modified oligonucleotides using template-dependent DNA ligation of shortmer fragments. Our approach is based on the fast...
Efficient assembly of very short oligonucleotides using T4 DNA …
We demonstrate the feasibility of simultaneous oligonucleotide phosphorylation and ligation and, as a proof of principle for DNA synthesis through the assembly of short oligonucleotides, we performed a hierarchical ligation procedure whereby octamers were combined to construct a target 128-bp segment of the beta-actin gene.
Annealing Oligonucleotides Protocol | IDT - Integrated DNA Technologies
2012年6月15日 · Ligation—if the double-stranded oligonucleotide product will be used in a ligation reaction, you may need to add 5’-phosphates to the strand ends. These can be added at the time of oligo synthesis (chemical phosphorylation; done by request) or anytime thereafter (before or after annealing) using polynucleotide kinase (enzymatic ...
Highly Parallelized Construction of DNA from Low-Cost Oligonucleotide …
2024年2月20日 · We propose an improvement on gene construction synthesis schemes using oligonucleotide (oligo) pools as sources of user-defined DNA sequences for decentralized gene construction through Golden Gate Assembly, following a simple three-step process.
Oligo annealing for ligation cloning — Galloway Lab Protocols …
2023年6月8日 · This protocol describes how to anneal and phosphorylate oligos to add short sequences to plasmids via ligation cloning. This could be useful to make new microRNA target sites or CRISPR guide RNAs, for instance.
A ligation-based single-stranded library preparation method to …
2019年12月27日 · Using synthetic DNA oligos and cfDNA, we demonstrate the efficiency and utility of this approach and compare with existing double-stranded and single-stranded approaches for library generation.
Ligation of a DNA or RNA oligo to the 3’ end of long ssRNA
2014年10月27日 · This protocol is for ligation of a DNA or RNA oligo donor with a 5'P to the 3'OH end of long ssRNA (> 0
Biocatalytic assembly of chemically modified oligonucleotides
2022年3月16日 · Inspired by a well-established biological construction of DNA and RNA strands [9], [10], [11], a therapeutic oligonucleotide can be split into two or more, shorter fragments, which can be ligated together using an RNA ligase. Shorter oligonucleotides are easier to synthesize chemically with a higher purity, which simplifies downstream ...