Total RNA was isolated using the RNeasy Plus Mini Kit (Qiagen). First-strand cDNA synthesis was performed using oligo(dT) primers (Promega) with the Qiagen Omniscript RT kit, according to the ...

The authors demonstrated cellular heterogeneity of companion cells (CCs) and also suggested the CC subpopulation that highly expressed the florigen gene FT. Based on this finding, they further ...

This important study reveals a critical role of the transcription factor NR2F2 in mouse fetal Leydig cell (FLC) differentiation. With elegantly carried out experiments, the authors provide compelling ...

Polymer synthesis, also called polymerization, is the process by which monomers (small molecules) are covalently bonded to form a (usually long) polymer chain or network. A facile and versatile ...

Biosynthesis can be exploited for chemical synthesis in vitro or in cells like Escherichia coli by combining substrates with enzymes using recombinant methods. Polymer/whole-cell hybrid catalysts ...