All specific primers must be supplied by the client, but we have available free of charge a few common sequencing primers: M13(-21)F and M13(-40)R. Please note: there are several primers called: M13F, ...

A Sanger sequencing reaction is run with a single primer. The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the ...

Figure 3: Before emPCR and sequencing, Genome Sequencer process–specific A and B adaptors must be appended to the cDNA copy of the small RNA. Prepare PCR templates with one tailed primer and one ...

Sequencing by capillary electrophoresis is highly sensitive to sample contamination. Potential contaminants include: proteins, RNA, chromosomal DNA, non-specific PCR products, residual salts, organic ...