The role of phosphorylation in regulating protein function and cellular signaling is perhaps one of the most important questions in biology. Classical methods like mobility-shift assays allow ...

A new study published in Molecular Biomedicine uncovers how post-translational modifications (PTMs) of fibrinogen—a key blood ...

Other technologies used to globally map phosphorylation in yeast by identifying novel kinase–substrate relationships include in vivo-based genetic approaches. In contrast to biochemical ...

"People have typically looked at 10 or 20 phosphorylation sites at a time," says Myers. "But because we're a mass spectrometry lab, we can use this technique to look at all phosphorylation—and ...

Mass spectrometry–based proteomics is still ... Researchers led by Steven Gygi at Harvard Medical School compared the phosphorylation status of proteins in yeast cells with or without active ...

TThe expression CMS is a single quadrupole mass spectrometer, equipped with ESI and APCI probes, switchable with minimal effort. The instrument has a mass range of m/z 10 to m/z 1,200, and a ...

Additionally, we employ quantitative mass spectrometry -based proteomics on assessing the protein abundance changes, as well as phosphorylation status of the tissues/cells. Furthermore, combining the ...