The role of phosphorylation in regulating protein function and cellular signaling is perhaps one of the most important questions in biology. Classical methods like mobility-shift assays allow ...

Mass spectrometry–based proteomics is still ... Researchers led by Steven Gygi at Harvard Medical School compared the phosphorylation status of proteins in yeast cells with or without active ...

Other technologies used to globally map phosphorylation in yeast by identifying novel kinase–substrate relationships include in vivo-based genetic approaches. In contrast to biochemical ...